| Popis: |
Detailed methods are described for preparation of bovine pancreatic ribonuclease (polyribonucleotide 2-oligonucleotidotransferase (cyclizing), EC 2.7.7.16) crystals of Modifications I and II of quality suitable for X-ray diffraction studies. The reasons for choice of solvent and buffers systems are discussed, especial regard being paid to the use of the solvent 2-methyl-2,4-pentanediol and to the application of preserving solutions as media for handling the crystals after growth. Some necessary precautions are specified. |
