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Background lumbar disc herniation (LDH) is a high-risk species in the world with serious harm. Traditional Chinese Tuina is the first choice in the treatment of LDH. In the past clinical studies, we have achieved good results in the treatment of LDH with the waist-rubbing method recorded in an ancient Chinese medical book of Qing Dynasty. Now we further explore its internal mechanism of action from the perspective of animal experiments. Waist-rubbing is a frictional heat-generating stimulus that acts on the surface of the skin. It does not relieve the compression in spinal canal, but can effectively eliminate the symptoms of LDH patients. This suggests that waist-rubbing may play a therapeutic role by interfering the autoimmunity of LDH. For a long time, the treatment of LDH mainly focused on how to relieve the mechanical compression caused by lumbar disc herniation, but little attention was paid to the autoimmune inflammation of LDH. In this study, from the point of view of regulating LDH's autoimmunity, the waist-rubbing method was taken as the treatment measure, and the lumbar strain rats in upright position were taken as the model. The neuroendocrine immunology mechanism of waist-rubbing method in treating LDH was revealed, which provided new ideas and scientific basis for Tuina clinical treatment of LDH, and also provided strong evidence for the theory of LDH's autoimmunity. Methods 30 SD rats were randomly divided into three groups of the blank, the model and the waist wiping. The LDH model of the forelimb-free standing rats was established. Each group was treated according to the corresponding method. The waist-rubbing group was treated once a day for 15 days. After the experiment, the blood and lumbar spinal cord samples were collected after rats been killed. The contents of the CRH(Corticotropin releasing hormone), ACTH(Adrenocorticotropic hormone), CORT(Cortisol), IL-17(Interleukin-17), IL-6(Interleukin-6), TGF-β1(Transforming growth factor-β1) and IL-10(Interleukin-10) in peripheral blood and the GRα(Glucocorticoid receptor α) and 5-HT1A(Five hydroxytryptamine 1A) receptor in L4-L6 segment of rat lumbar spinal cord were detected by ELISA(Enzyme-linked immunoSorbent assay). Results Compared with the blank group, the contents of CRH, ACTH, CORT, GRα, 5-HT1A receptor, TGF-β1 and IL-10 in the model group were significantly decreased (P P α, 5-HT1A receptor, TGF-β1 and IL-10 in the waist-rubbing group were significantly increased (P P |
