Overexpression of full-length and partial DREB2A enhances soybean drought tolerance

Autor: Juliana Marcolino-Gomes, André Luís Hartmann Caranhoto, Liliane Marcia Mertz-Henning, Kazuko Yamaguchi-Shinozaki, Kazuo Nakashima, Silvana Regina Rockenbach Marin, Juliane Prela Marinho, Alexandre Lima Nepomuceno, José Salvador Simonet Foloni, Carlos Lásaro Pereira de Melo, Satoshi Kidokoro, Norihito Kanamori, Renata Fuganti Pagliarini, Mayla Daiane Correa Molinari, Maria Cristina Neves Oliveira, Junya Mizoi
Rok vydání: 2021
Předmět:
Zdroj: Agronomy Science and Biotechnology. 8:1-21
ISSN: 2359-1455
DOI: 10.33158/asb.r141.v8.2022
Popis: Soybean is an important commodity worldwide. Abiotic conditions can adversely disturb crop growth and final yield. The transcription factor Dehydration-Responsive Element-Binding Proteins 2 (DREB2) act as a regulator of drought-responses. This study aimed to characterize soybean plants genetically modified with GmDREB2A;2 FL and GmDREB2A;2 CA for molecular, physiological, and agronomic responses, at different developmental periods. Results showed that seedlings from GmDREB2A;2 FL event presented lower growth reduction under osmotic treatment during germination. The GmDREB2A;2 FL and GmDREB2A;2 CA events showed improved performance in experiments of water deficit imposed in the vegetative period and higher rates in physiological parameters. In the reproductive period, there was a trend of higher yield compounds in GM GmDREB2A;2 FL event when compared to other genotypes and treatments. It was suggested that GmDREB2A;2 FL event presented superior performance due to the higher expression levels of the cisgene and drought-induced genes.
Databáze: OpenAIRE