| Autor: |
Jianjun Sun, Jia-yin SHEN, Feng-di ZHANG, Ren-fang ZHANG, Li LIU, Yin-zhong SHEN, Jiang-rong WANG, Tang-kai QI, Zhen-yan WANG, Yang TANG, Wei SONG, Jun CHEN, Li-qian GUAN, Mei-yan SUN, Shu-ye ZHANG, Hong-zhou LU |
| Rok vydání: |
2020 |
| DOI: |
10.21203/rs.2.22572/v1 |
| Popis: |
Background Among Chinese HIV-infected patients we assessed the impact of different KIR-HLA combinations on the viral set point levels, CD4/CD8 ratios and plasma soluble CD14, all of which are risk factors for the prognosis of HIV patients. Methods Peripheral blood samples were collected from newly diagnosed HIV-infected patients who were treatment naive, and processed according to the study design. Participants were selected according to the inclusive criteria and their clinical data and other demographic information were recorded. The genomic DNA of the host was extracted from the whole blood samples, and the KIR genotyping was performed by sequence specific primer amplification (PCR-SSP); the HLA genotyping was performed by sequence analysis (PCR-SBT); and the HLA-KIR genotyping and combination information were obtained. Results In China, the distribution of KIR genotypes in HIV patients was similar to that of KIR genotypes in people without HIV infection; the baseline HIV level in patients with HLA-C1+/KIR2DL3+ background was significantly higher than that of HLA-C1+/KIR2DL3-(4.34 log10 copies/ml v.s 3.72 log10 copies/ml, P=0.02); the baseline CD4/CD8 ratio of HLA-C1+/KIR2DL3+ was significantly lower than that of HLA-C1+/KIR2DL3-(0.33 v.s 0.56, P=0.02). The plasma soluble CD14 level at baseline was significantly lower in HLA-C1+/KIR2DL3- infected persons than that of HLA-C1+/KIR2DL3+ (P=0.03). Conclusions NK cells may affect the viral set point and host immune status in HIV-infected patients through the combination of KIR molecules and their corresponding ligand HLA, thus affecting the prognosis of HIV infection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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