Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques

Autor: Catherine R. Cabrera, Paul Yager
Rok vydání: 2001
Předmět:
Zdroj: ELECTROPHORESIS. 22:355-362
ISSN: 1522-2683
0173-0835
DOI: 10.1002/1522-2683(200101)22:2<355::aid-elps355>3.0.co;2-c
Popis: A novel method for the concentration of bacterial solutions is presented that implements electrokinetic techniques, zone electrophoresis (ZE) and isoelectric focusing (IEF), in a microfluidic device. The method requires low power (< 3e-5 W) and can be performed continuously on a flowing stream. The device consists of two palladium electrodes held in a flow cell constructed from layers of polymeric film held together by a pressure-sensitive adhesive. Both ZE and IEF are performed with carrier-free solutions in devices in which the electrodes are in intimate contact with the sample fluid. IEF experiments were performed using natural pH gradients; no carrier ampholyte solution was required. Experiments performed in buffer alone resulted in significant electroosmotic flow. Pretreatment of the sample chamber with bleach followed by a concentrated solution of cationic detergent effectively suppressed electroosmotic flow.
Databáze: OpenAIRE
Externí odkaz: