Sequence Homology of Two Indian Isolates of Cucumber Mosaic Virus at N-terminal Amino Acid Sequences of the Coat Protein Gene with CMV Strains

Autor: S. K. Raj, K. M. Srivastava, Q. M. R. Haq, Bajrang Singh
Rok vydání: 1995
Předmět:
Zdroj: Journal of Plant Biochemistry and Biotechnology. 4:77-80
ISSN: 0974-1275
0971-7811
DOI: 10.1007/bf03262957
Popis: The cDNAs representing coat protein gene (RNA4) of CMV-CR (Dianthus barbatus strain) and CMV-P (Physalis minima strain) were synthesized using random primers and cloned into Bluescript II KS (+) phagemid at Sma I site. The clones were partially sequenced and compared with published nucleotide sequences of selected CMV-strains. Comparison of 249 nucleotide sequences of 5′ coding region of CP gene and their putative products revealed the possibility of placing the strains into sub-group I of CMV.
Databáze: OpenAIRE