Comparison of selective agents for use with the selectable marker gene bar in maize transformation

Autor: Briana K. Dennehey, Charles L. Armstrong, Colleen Ford-Santino, William L. Retersen, Mark E. Pajeau
Rok vydání: 1994
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture. 36:1-7
ISSN: 1573-5044
0167-6857
DOI: 10.1007/bf00048308
Popis: The effectiveness of four phosphinothricin (PPT)-based selective agents were evaluated for use in maize transformation: glufosinate, bialaphos, Basta® and Herbiace®. Glufosinate and its commercial formulation, Basta®, were less effective in controlling growth of non-transgenic corn callus than the tripeptide, bialaphos, or its commercial formulation, Herbiace®. Addition of 25 mM l-proline had no significant effect on selection when using bialaphos. However, when l-proline was included with the selective agent glufosinate, selection was inhibited and callus growth was enhanced. At four weeks, callus growth on 0.3, 1.0 and 3.0 mg l-1 glufosinate in the presence of proline was 76, 43, and 21% of control growth, respectively, and in the absence of proline was only 32, 9, and 6% of control growth. Optimized selection protocols for Basta® and bialaphos yielded comparable numbers of transformants. Using these protocols, fertile transgenic plants were regenerated from transformed callus cultures.
Databáze: OpenAIRE
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