Bubble inducing cell lysis in a sessile droplet

Autor:	Charles Wai Chung Ma, Adrian Neild, Ninnuja Sivanantha, Jue Nee Tan
Rok vydání:	2014
Předmět:	Jet (fluid) Chromatography Lysis Physics and Astronomy (miscellaneous) Chemistry Bubble Substrate (chemistry) complex mixtures Cell membrane medicine.anatomical_structure Sessile droplet medicine Nucleic acid Biophysics Sample preparation
Zdroj:	Applied Physics Letters. 104:103704
ISSN:	1077-3118 0003-6951
DOI:	10.1063/1.4868407
Popis:	Cell lysis is a key sample preparation stage in many biomedical studies as DNA extraction and classification require the use of the nucleic acid and proteins released upon decomposition of a cell membrane. We present an effective method of lysing cells suspended in a microliter droplet placed on a super-hydrophobic surface. When a bubble, injected into the sessile droplet, subsequently ruptures, a rapidly moving fluid jet is formed. In this work, cells that are transported within this fluid jet are captured on a separate hydrophilic substrate and are shown to have been lysed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6dc9134f678d6f9c463994f8294e40ef https://doi.org/10.1063/1.4868407 Zobrazit plný text záznamu