Inflammation‐mediated deacetylation of the ribonuclease 1 promoter via histone deacetylase 2 in endothelial cells
Autor: | Nicoletta Scheller, Bernd Schmeck, Silke Leiting, Silvia Fischer, Evelyn Vollmeister, Klaus T. Preissner, Katrin Bedenbender |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
biology Histone deacetylase 2 Chemistry Biochemistry Cell biology Proinflammatory cytokine Chromatin 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Histone Acetylation Genetics biology.protein Transcriptional regulation Histone deacetylase Molecular Biology Chromatin immunoprecipitation 030217 neurology & neurosurgery Biotechnology |
Zdroj: | The FASEB Journal. 33:9017-9029 |
ISSN: | 1530-6860 0892-6638 |
Popis: | Ribonuclease 1 (RNase1) is a circulating extracellular endonuclease that regulates the vascular homeostasis of extracellular RNA and acts as a vessel- and tissue-protective enzyme. Upon long-term inflammation, high amounts of proinflammatory cytokines affect endothelial cell (EC) function by down-regulation of RNase1. Here, we investigated the transcriptional regulation of RNase1 upon inflammation in HUVECs. TNF-α or IL-1β stimulation reduced the expression of RNase1 relative to the acetylation state of histone 3 at lysine 27 and histone 4 of the RNASE1 promoter. Inhibition of histone deacetylase (HDAC) 1, 2, and 3 by the specific class I HDAC inhibitor MS275 abolished the TNF-α- or IL-1β-mediated effect on the mRNA and chromatin levels of RNase1. Moreover, chromatin immunoprecipitation kinetics revealed that HDAC2 accumulates at the RNASE1 promoter upon TNF-α stimulation, indicating an essential role for HDAC2 in regulating RNase1 expression. Thus, proinflammatory stimulation induced recruitment of HDAC2 to attenuate histone acetylation at the RNASE1 promoter site. Consequently, treatment with HDAC inhibitors may provide a new therapeutic strategy to stabilize vascular homeostasis in the context of inflammation by preventing RNase1 down-regulation in ECs.-Bedenbender, K., Scheller, N., Fischer, S., Leiting, S., Preissner, K. T., Schmeck, B. T., Vollmeister, E. Inflammation-mediated deacetylation of the ribonuclease 1 promoter via histone deacetylase 2 in endothelial cells. |
Databáze: | OpenAIRE |
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