Immobilization of Penicillium solitum lipase on the carbon fiber material 'Dnepr-MN'

Autor:	G. I. Bondarenko, I. I. Romanovskaya, T. I. Davidenko
Rok vydání:	2008
Předmět:	Pharmacology chemistry.chemical_classification Chromatography biology Immobilized lipase Penicillium solitum Matrix (chemical analysis) Enzyme chemistry Desorption Drug Discovery biology.protein medicine Fiber Lipase Activated carbon medicine.drug
Zdroj:	Pharmaceutical Chemistry Journal. 42:360-362
ISSN:	1573-9031 0091-150X
Popis:	A method for immobilizing Penicillium solitum lipase (the medicinal agent Solizyme) on the activated carbon fiber material “Dnepr-MN” is proposed; using a matrix with total pore volumes of 0.41–1.3 cm3/g, the method allows the enzyme:matrix ratio (1:30–1:60) to be controlled, yielding preparations with a specified lipolytic activity (20–40 LU/mg of agent) at quantitative yield (91–97%). Studies of the pH and temperature stability, functioning in acid media, and desorption demonstrated stabilization and prolongation of the lipolytic activity of the immobilized lipase.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6d9d3e48589e5c234ee591c21eb5e6c9 https://doi.org/10.1007/s11094-008-0127-5 Zobrazit plný text záznamu Plný text ve formátu PDF