A new biochemical dye molecule and nuclear stain formulation in histotechnology diagnostics as an alternative to hematoxylin
| Autor: | Mehmet Budak, Gurer G. Budak |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Pathology Histology Auramine-rhodamine stain 010304 chemical physics H&E stain Diagnostic test Biology 010402 general chemistry Nuclear staining 01 natural sciences Stain 0104 chemical sciences Staining Medical Laboratory Technology 0103 physical sciences medicine Histopathology Dye molecule Anatomy |
| Zdroj: | Journal of Histotechnology. 38:113-121 |
| ISSN: | 2046-0236 0147-8885 |
| DOI: | 10.1179/2046023615y.0000000009 |
| Popis: | In this study, a novel molecule, a nucleotide permanent marker for nuclear staining from Papaver rhoeas (common poppy), and a sustainable stain formulation is disclosed. The molecule appears to penetrate tissue layers aggressively, and specifically attaches deoxyribonucleic acid nucleotides, staining the nucleus and revealing microscopic details. Various hematoxylin nuclear stain formulations have been presented for diagnostics, in the relative absence of viable alternatives. Millions of surgical microscopic diagnostic tests (2.5–3 million slides every day) are processed with hematoxylin, throughout the world. Apparently, hematoxylin is the most commonly used nuclear stain for histopathology diagnostics in the world, although the present cost – financial and ecological – of hematoxylin protocols is substantial and not well appreciated, even by pathologists and histologists. The origin of the hematoxylin is the Logwood tree (Haematoxylum campechianum), a species indigenous to the tropical rainforests of Ce... |
| Databáze: | OpenAIRE |
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