Blood compatible heteratom-doped carbon dots for bio-imaging of human umbilical vein endothelial cells
| Autor: | Jiayi Cui, Waleed Ali Khan, Lin He, Jian Zhong, Kaixuan Yu, Chaoxin Xiao, Lulu Cai, Chen Xinmian, Miaoran Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Chemistry
chemistry.chemical_element Quantum yield 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease 01 natural sciences Fluorescence Hemolysis Umbilical vein 0104 chemical sciences In vivo medicine Biophysics 0210 nano-technology Biological imaging Cytotoxicity Carbon |
| Zdroj: | Chinese Chemical Letters. 31:769-773 |
| ISSN: | 1001-8417 |
| DOI: | 10.1016/j.cclet.2020.01.007 |
| Popis: | Carbon dots have unique advantages in biological applications owing to their excellent optical properties. However, the biosafety evaluation of carbon dots has limitations owing to cytotoxicity in vitro, and there is little pre-safety evaluation before in vivo and clinical applications. Whether the carbon dots are or not suitable for applications in vivo, evaluation analysis can be made based on hemolysis and changes in erythrocyte morphology. In this work, a green fluorescent N, S-doped carbon dots (N, S-CDs) were obtained by hydrothermal method, tobias acid, and m-phenylenediamine as precursors. N, S-CDs not only possessed excellent dispersibility, uniform particle size, high quantum yield (37.2%) and stable photoluminescence property but also retain their photostability and strong fluorescence intensity in the acid/alkaline solutions, different ionic strengths (NaCl) and under 365 nm UV illumination. Moreover, the N, S-CDs displayed low cytotoxicity and high cellular uptake efficiency in human umbilical vein endothelial cells (HUVEC) and excellent blood compatibility to the erythrocyte. It is foreseeable that N, S-CDs could be further studied as a promising biological imaging agent in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect