Measurement of two-photon-absorption spectra through nonlinear fluorescence produced by a line-shaped excitation beam

Autor:	Elton Hasani, Luca Tartara, D. Tomassini, A. Tomaselli, Jacopo Parravicini
Rok vydání:	2018
Předmět:	0301 basic medicine Histology Materials science Photon business.industry Detector Physics::Optics Optical power 01 natural sciences Fluorescence Signal Two-photon absorption Spectral line Pathology and Forensic Medicine 010309 optics 03 medical and health sciences 030104 developmental biology Optics 0103 physical sciences business Absorption (electromagnetic radiation)
Zdroj:	Journal of Microscopy. 270:210-216
ISSN:	0022-2720
DOI:	10.1111/jmi.12675
Popis:	We propose an innovative experimental approach to estimate the two-photon absorption (TPA) spectrum of a fluorescent material. Our method develops the standard indirect fluorescence-based method for the TPA measurement by employing a line-shaped excitation beam, generating a line-shaped fluorescence emission. Such a configuration, which requires a relatively high amount of optical power, permits to have a greatly increased fluorescence signal, thus avoiding the photon counterdetection devices usually used in these measurements, and allowing to employ detectors such as charge-coupled device (CCD) cameras. The method is finally tested on a fluorescent isothiocyanate sample, whose TPA spectrum, which is measured with the proposed technique, is compared with the TPA spectra reported in the literature, confirming the validity of our experimental approach.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6d843f092a59cc0d7ca57a0f969452aa https://doi.org/10.1111/jmi.12675 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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