Synthesis of a New Vitamin D3 Hapten and Its Protein Conjugates
| Autor: | T. V. Mirantsova, Vladimir A. Khripach, A. P. Savachka, A. L. Sauchuk, A. G. Pradko, R. P. Litvinovskaya |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Vitamin Ergosterol Chromatography 030102 biochemistry & molecular biology biology Chemistry Organic Chemistry Conjugated system Biochemistry Horseradish peroxidase 03 medical and health sciences chemistry.chemical_compound biology.protein Bioorganic chemistry Bovine serum albumin Hapten Conjugate |
| Zdroj: | Russian Journal of Bioorganic Chemistry. 44:778-786 |
| ISSN: | 1608-330X 1068-1620 |
| DOI: | 10.1134/s1068162018050102 |
| Popis: | The synthesis of 25-(1-carboxymethoxy)-imino-3(S)-hydroxy-9,10-seco-27-norcholesta-5(Z),7(E),10(19)-triene, a new hapten of vitamin D3, has been carried out in ten steps from ergosterol. The hapten was conjugated to horseradish peroxidase and bovine serum albumin. Antibodies to 25-hydroxyvitamin D3 were obtained using the synthesized conjugates. An immunochemical system was developed for the quantitative determination of 25-hydroxyvitamin D3. |
| Databáze: | OpenAIRE |
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