Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction
| Autor: | Jerre W. Mohler |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Ecology
technology industry and agriculture Anatomy Management Monitoring Policy and Law Aquatic Science Biology Fluorescence Life stage Calcein Andrology chemistry.chemical_compound chemistry polycyclic compounds lipids (amino acids peptides and proteins) heterocyclic compounds sense organs Ecology Evolution Behavior and Systematics |
| Zdroj: | North American Journal of Fisheries Management. 23:1108-1113 |
| ISSN: | 1548-8675 0275-5947 |
| DOI: | 10.1577/m02-143 |
| Popis: | Calcified tissues of Atlantic salmon in both larval and postscale developmental life stages became labeled with the fluorochrome dye known as calcein in 7 min by sequential immersion in salt and calcein solutions. I introduce the term “osmotic induction” to describe this process. At 47 d posttreatment, fish that had been exposed to osmotic induction of calcein as nonfeeding larvae exhibited mortality and growth equivalent to that of nonexposed fish (P < 0.05). Atlantic salmon parr (at 5 months posthatch) exposed to osmotic induction of calcein exhibited no mortality during a 120-d posttreatment period. In addition to inducing a calcein mark on fin rays of all larvae and parr, the procedure also produced a distinct calcein mark on scales of parr. A second exposure of parr to osmotic induction 90 d after the first produced a conspicuous 2-band pattern on scales which corresponded to the two separate marking events. Calcein marks are invisible to the unaided eye but were observed without sacrificing... |
| Databáze: | OpenAIRE |
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