| Autor: |
R. Gayatri Devi, Selvaraj Jayaraman, A. Jothi Priya, K. Swetha |
| Rok vydání: |
2022 |
| Zdroj: |
Journal of Pharmaceutical Research International. :18-24 |
| ISSN: |
2456-9119 |
| DOI: |
10.9734/jpri/2022/v34i27b36001 |
| Popis: |
Introduction: Citrullus colocynthis is a desert plant of the cucurbitaceae family. C. colocynthis grows in many countries with semi dry lands and that seeds are rich in oil which can encourage their sustainable agriculture to produce an economic biodiesel with a competitive price. Apoptosis, also termed as programmed cell death this is a key regulator of tissue homeostasis. Aim of the Study: To study the induction of bax and activation of caspases hydro ethanolic leaf extract of Citrullus colocynthis (L)- mediated apoptosis in human liver cancer cells Hep G2. Materials and Methods: In the present study, a human liver cancer cell line was procured from the National Centre for Cell science (NCCS), Pune, India. The cytotoxic potential of plant extract was analysed by MTG assay. mRNA expression of BaxmRNA and caspase-mRNA were analysed by Real-Time PCR using gene specific primers and a comparative CT method was used to analyse expression of genes. The data were analysed statistically using Graph pad prism software Version 5. Results: MTT analysis showed that Citrullus colocynthis a significant increase in proliferation of human liver cancer cells (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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