Effect of Dipeptides on In vitro Maturation, Fertilization and Subsequent Embryonic Development of Porcine Oocytes

Autor: Inho Choi, Quzi Sharmin Akter, M.A.M. Yahia Khandoker, Hirotada Tsujii, K. M. A. Tareq
Rok vydání: 2013
Předmět:
Zdroj: Asian-Australasian Journal of Animal Sciences. 26:501-508
ISSN: 1976-5517
1011-2367
Popis: The effects of amino acids and dipeptides on in vitro production of porcine embryos and accumulation of ammonia in culture medium during developmental stages were examined in this study. The maturation, fertilization and development of embryonic cultures were performed in modified Tissue culture medium (mTCM)-199 supplemented with 10% (v/v) porcine follicular fluid, modified Tyrode's albumin lactate pyruvate (mTALP) medium, and modified North Carolina State University (mNCSU)-23 medium, respectively. In addition, amino acids and dipeptides of different concentrations and combinations were used to treat the embryos. The addition of L-alanyl-L-glutamine (AlnGln)L-glycyl-L-glutamine (GlyGln) significantly (p
Databáze: OpenAIRE