Exogenous thrombospondin stimulates the proliferation of non-thrombospondin-producing cells
| Autor: | Tetsushi Kinugasa, R Beppu, S Sendo, T Kurohiji, Takayuki Shirakusa, T Hosokawa, Y. Yamashita, G Tuszynski, Tetsuo Shinohara |
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| Rok vydání: | 1998 |
| Předmět: |
endocrine system
Cancer Research medicine.medical_specialty Thrombospondin Cell growth Immunoelectron microscopy Cell virus diseases Cell cycle Biology Molecular biology Endocrinology medicine.anatomical_structure Oncology immune system diseases Cell culture Internal medicine Thrombospondin 1 medicine Thrombospondins |
| Zdroj: | International Journal of Oncology. |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.13.2.355 |
| Popis: | The effects of thrombospondin (TSP) on the proliferation of two different human carcinoma cell lines (KIM-1 and CW-2) were investigated. The characterization of these two carcinoma cells by immunohistochemistry using anti-TSP antibody and anti-TSP-receptor antibody showed that the KIM-1 had TSP-receptors and TSP, while the CW-2 had only TSP-receptors. The addition of exogenous TSP (10 or 20 microg/ml) to culture medium stimulated the cell proliferation of CW-2 but not that of KIM-1. The cell count for CW-2 was increased dosage-dependently from 10.3 0.6x104/ml at zero TSP concentration to 12.9 0.6x104/ml at 10 microg/ml TSP concentration and to 14.7 0. 4x104/ml at 20 microg/ml TSP (each p |
| Databáze: | OpenAIRE |
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