GC/MS analysis and anti-inflammatory effect of leaf extracts from Hibiscus syriacus through inhibition of NF-κB and MAPKs signaling in LPS-stimulated RAW264.7 macrophages
| Autor: | Yeongyeong Kang, Hae Yun Kwon, Youngki Park, Hyun Ji Eo, Gwang Hun Park, Som Da Kim |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Messenger RNA biology medicine.drug_class p38 mitogen-activated protein kinases NF-κB Plant Science biology.organism_classification 01 natural sciences Molecular biology Anti-inflammatory 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry medicine Ethyl palmitate Phosphorylation Hibiscus syriacus Signal transduction 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Biotechnology Reports. 14:539-546 |
| ISSN: | 1863-5474 1863-5466 |
| DOI: | 10.1007/s11816-020-00628-3 |
| Popis: | Hibiscus syriacus (H. syriacus), which belongs to Malvaceae, is a perennial herb widely distributed in East Asia. In this study, we investigated the anti-inflammatory effects and its potential molecular mechanisms of leaves extracted with 70% ethanol of H. syriacus (HS-L) at the mRNA and protein level when RAW264.7 macrophages were stimulated by LPS. HS-L dose dependently (0, 25, 50, 100 μg/mL) reduced NO production by inhibiting iNOS, COX-2, and IL-β expression in LPS-induced RAW264.7 macrophages. HS-L decreased the LPS-stimulated degradation and phosphorylation of IkB-a. Moreover, treatment with HS-L suppressed LPS-induced DNA binding of NF-kB and nuclear translocation of p65. Furthermore, HS-L suppressed the phosphorylation of ERK1/2 and p38. HS-L was analyzed to contain compounds such as 2-pentadecanone, methyl palmitate, and ethyl palmitate in GC/MS analysis. These results suggested that HS-L could utilize anti-inflammatory activity by inhibiting NF-κB and MAPKs signaling pathways and that HS-L could be used as a natural anti-inflammatory agent. |
| Databáze: | OpenAIRE |
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