Conversion of Agroindustrial Wastes to Rhamnolipid by Enterobacter sp. UJS-RC and Its Role against Biofilm-Forming Foodborne Pathogens
| Autor: | Poorna Chandrika Sabapathy, Guoyan Zhang, Hossain M. Zabed, Xianghui Qi, Junhua Yun, Yuvaraj Ravikumar, Radhika Chandankere |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Reactive oxygen species biology Chemistry Pseudomonas aeruginosa 010401 analytical chemistry Rhamnolipid Biofilm General Chemistry medicine.disease_cause biology.organism_classification 01 natural sciences Corn steep liquor 0104 chemical sciences chemistry.chemical_compound Staphylococcus aureus medicine Enterobacter sp Food science General Agricultural and Biological Sciences Bacteria 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 68:15478-15489 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Rhamnolipid is the main group of biosurfactants predominantly produced by Pseudomonas aeruginosa, a ubiquitous and opportunistic pathogen, which limits its large-scale exploitation. Thus, cost-effective rhamnolipid production from a newly isolated nonpathogenic Enterobacter sp. UJS-RC was investigated. The highest rhamnolipid production (4.4 ± 0.2 g/L) was achieved in a medium constituting agroindustrial wastes (sugarcane molasses and corn steep liquor) as substrates. Rhamnolipid exhibited reduced surface tension to 72-28 mN/m with an emulsification index of 75%. The structural analyses demonstrated the presence of methoxyl, carboxyl, and hydroxyl groups in rhamnolipid. Mass spectra indicated eight rhamnolipid congeners, where dirhamnolipid (m/z 650.01) was the dominant congener. Rhamnolipid inhibited biofilm formation of Staphylococcus aureus in a dose-dependent manner, supported by scanning electron microscopy disclosing the disruption of the microcolony/exopolysaccharide matrix. Rhamnolipid's ability to generate reactive oxygen species has thrown light on the mechanism through which the killing of test bacteria may occur. |
| Databáze: | OpenAIRE |
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