Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme
| Autor: | Edzna S. Garcia, Thao M. Xiong, Steven C. Zimmerman, Abygail Lifschitz |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics Tandem Organic Chemistry Radical polymerization technology industry and agriculture chemistry.chemical_element Bioengineering Polymer Fluorine-19 NMR Biochemistry Combinatorial chemistry Fluorescence spectroscopy Catalysis Ruthenium chemistry Proton NMR |
| Zdroj: | Polymer Chemistry. 12:6755-6760 |
| ISSN: | 1759-9962 1759-9954 |
| Popis: | A Ru-containing single chain nanoparticle (SCNP) was prepared in three steps using radical polymerization of pentafluorophenylacrylate, post-polymerization functionalization with three different alkylamines, and coordination of Ru. The polymer was characterized by 1H NMR, 19F NMR, UV-vis, and DLS. The catalytic activity of the Ru-SCNP for Ru-catalyzed cleavage of allylcarbamates was evaluated by fluorescence spectroscopy and a higher percent conversion and initial rate of reaction was observed when compared to that of the free catalyst in buffer and cell media. The catalytic SCNP was also shown to perform tandem catalysis with β-galactosidase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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