A new class of 3′-modified nucleotides as chain-terminators for DNA-sequencing
| Autor: | Konrad Faulstich, Siegfried Brandtner, Joachim W. Engels, Christian Griesinger, Renate Konrad, Mathias Kurt Herrlein |
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| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
DNA clamp Chemistry Organic Chemistry Clinical Biochemistry Pharmaceutical Science Maxam–Gilbert sequencing T7 DNA polymerase Biochemistry Molecular biology DNA sequencing chemistry.chemical_compound Drug Discovery Molecular Medicine Nucleotide Fluorescein Primer (molecular biology) Thymidine Molecular Biology |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 4:1975-1978 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/s0960-894x(01)80546-1 |
| Popis: | We describe the use of 3′-modified thymidine nucleotides as potent chain terminators in Sanger's DNA sequencing reactions by application of T7 DNA polymerase and Mn 2+ -ions. These nucleotides contain fluorescein which is linked directly or by mono-, di- and tetraglycine units to the 3′-position of a 3′-amino-2′-3′-dideoxyribose. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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