Virus isolation and molecular epidemiology of highly pathogenic avian influenza A(H5N8) from an outbreak in free-ranging wild birds, India 2016
Autor: | M. D. Venkatesha, Poorvi Reddy, Beechagondahalli Papanna Shivashankar, Thushara Bindu Kv, S. M. Byregowda, Byadarahalli Puttaswamy Shankar, Kantharajapura Ramanna Ramesha, B. M. Chandranaik, Poojappa Nandini, Methuku Sobha Rani |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Molecular epidemiology viruses Embryonated Wildlife virus diseases Outbreak Management Monitoring Policy and Law Biology medicine.disease_cause Virology Virus Influenza A virus subtype H5N1 03 medical and health sciences 030104 developmental biology Phylogenetics TaqMan medicine Ecology Evolution Behavior and Systematics Nature and Landscape Conservation |
Zdroj: | European Journal of Wildlife Research. 64 |
ISSN: | 1439-0574 1612-4642 |
Popis: | We report the molecular epidemiology of highly pathogenic avian influenza (HPAI) virus involved in an outbreak causing death in free-ranging wild birds at Mysore, Karnataka state of India. The virus was typed as HPAI A(H5N8) by conventional and TaqMan probe based real-time PCR assays. Six isolates of HPAI virus were recovered in 9-day-old embryonated chicken eggs. Haemagglutinin gene-based phylogeny of virus isolates showed > 99.9% nucleotide sequence identity with HPAI A(H5N8) isolates from migratory birds and domestic poultry from China and Korea indicating either these wild birds have routed their migration through Korea and/or eastern China or these dead birds must have directly or indirectly contacted with wild birds migrating from Eastern China and/or Korean regions. The study emphasises the role of migratory wild birds in spread of HPAI across the globe. |
Databáze: | OpenAIRE |
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