Magnetic microparticle-based multimer detection system for the electrochemical detection of prion oligomers in sheep using a recyclable BDD electrode
| Autor: | Kuntaek Lim, Daisy Bougard, Christiane Segarra, Amel Sbartai, Sungmin Kang, Seong Soo A. An, Nicole Jaffrezic-Renault, Chantal Fournier-Wirth |
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| Rok vydání: | 2021 |
| Předmět: |
Boron doped diamond
Materials science Plasma samples 010401 analytical chemistry Inorganic chemistry 02 engineering and technology Electrochemical detection 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry law.invention law Electrode Fluidics Microparticle 0210 nano-technology human activities Spectroscopy Chemiluminescence |
| Zdroj: | Microchemical Journal. 164:106089 |
| ISSN: | 0026-265X |
| DOI: | 10.1016/j.microc.2021.106089 |
| Popis: | There is a strong need of sensitive and accurate diagnostic tests of infectious prion proteins (PrPSc) in pre-symptomatic individuals and animals. This work associates, for the first time, the magnetic microparticle multimer detection system (MDS) with a recyclable boron doped diamond (BDD) electrode for the electrochemical detection of PrPSc in plasma samples from scrapie-infected sheep with clinical signs. The electrochemical results are in agreement with the chemiluminescence results. The main advantages of the electrochemical fluidic system are its recyclability and the use of low quantity of functional magnetic beads. |
| Databáze: | OpenAIRE |
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