Protein Synthesis during Neural and Epidermal Differentiation in Cynops Embryo. (Cynops pyrrhogaster/protein synthesis/neural induction/concanavalin A/epidermal differentiation)

Autor:	Takashi Takabatake, Kenzo Takata, Tadashi C. Takahashi, Kazuhito Takeshima
Rok vydání:	1991
Předmět:	Cynops Genetics animal structures biology Embryogenesis Ectoderm Cell Biology biology.organism_classification Blastula Cell biology Gastrulation medicine.anatomical_structure Neurula embryonic structures medicine Cynops pyrrhogaster Neural plate Developmental Biology
Zdroj:	Development, Growth and Differentiation. 33:277-282
ISSN:	1440-169X 0012-1592
DOI:	10.1111/j.1440-169x.1991.00277.x
Popis:	Two-dimensional gel electrophoresis was used to analyze protein synthesis in relation to neural and epidermal differentiation in Cynops pyrrhogaster embryo. Various regions of embryos at different developmental stages, from late morula to early neurula stages, were excised, radiolabelled with 35S-methionine, and the pattern of protein synthesis were compared. The following four types of protein spots were observed: (1) six proteins synthesized characteristically in the epidermal region of the embryo after gastrulation, (2) two proteins synthesized in both epidermal and endodermal regions, but not in other regions, after gastrulation, (3) a protein first detected at early blastula stage, of which expression was nearly constant in presumptive epidermis region but declined in the other regions, (4) the candidate for neural plate specific protein synthesized at a very high level in ectoderm explants treated with concanavalin A, a substance which evokes neural induction.
Databáze:	OpenAIRE
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