A reversible microarray immobilization strategy based on thiol-quinone reaction
| Autor: | Shao Q. Yao, Yusheng Xie, Jingdong Luo, Ling Feng, Hongyan Sun, Jie Zhang, Jiang Xia, Yi Feng, Ping Wang, Qingxin Chen |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
chemistry.chemical_classification
Microarray Chemistry 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry 0104 chemical sciences Quinone chemistry.chemical_compound Thioether Thiol Gene chip analysis Molecule 0210 nano-technology Biochip Linker |
| Zdroj: | Chinese Chemical Letters. 33:213-216 |
| ISSN: | 1001-8417 |
| Popis: | Microarray technology has been widely applied in biomedical research. The key to microarray study is to develop efficient immobilization method. In this study, we designed a new reversible microarray immobilization method based on thiol-quinone reaction. A quinone-functionalized slide was fabricated through H2O2 treatment of dopamine-coated slides. Various thiol-containing molecules can be anchored onto the quinone-functionalized slides via thioether linker, which could be cleaved under H2O2 treatment to regenerate quinone groups on the surface. The highly versatile approach can be widely used for immobilization of various thiol-containing molecules. |
| Databáze: | OpenAIRE |
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