In vitro characterization of angiotensin mediated ACTH release
| Autor: | D O Sobel, R E Johnsonbaugh |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
endocrine system
medicine.medical_specialty IBMX Chemistry Cyproheptadine Cyclase Angiotensin II chemistry.chemical_compound Endocrinology Internal medicine Pediatrics Perinatology and Child Health Renin–angiotensin system cardiovascular system medicine ACTH receptor sense organs Prostaglandin E2 hormones hormone substitutes and hormone antagonists Intracellular circulatory and respiratory physiology medicine.drug |
| Zdroj: | Pediatric Research. 15:1563-1563 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-198112000-00166 |
| Popis: | We recently demonstrated that angiotensin II(AII)directly mediates in vitro ACTH secretion in rat pituitary cells.To investigate the structure activity relationship of the All molecule and intra-cellular events involved in All mediated ACTH release we studied: 1)the ACTH stimulatory activity of the All fragments AII(2-8),AII (3-8)and AII(4-8) 2)the effect of isobutyl-3-methylxanthine(IBMX)an adenyl cyclase inhibitor,prostaglandin E2 (PGE2)and cyproheptadine on All mediated ACTH release.Anterior rat pituitaries were enzymatically dispersed in trypsin/DNAase solution and placed in mono-layer cell cultures.After 3 days of incubation,the cells were washed and incubated for 4 hrs in test media which was later asayed for ACTH by RIA.AII(10nM)elicited a 150-180% increase of ACTH secretion over control cells.A declining ACTH releasing activity was demonstrated with each consecutive C terminal aminoacid deletion from AII.AII with IBMX(1mM)released more ACTH over its control (IBMX alone) than the ACTH released by All over its control(p AII(2-8)>AII (3-8)>AII(4-8).IBMX potentiates,PGE2 inhibits,and cyproheptadine has no effect on AII mediated ACTH release.Thus the C terminal aminoacids of AII are important to its ACTH stimulatory activity.It is suggested that higher intracellular C'AMP sensitized AII mediated ACTH release and PGE2 may be a factor in this process. |
| Databáze: | OpenAIRE |
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