Gastrodin causes vasodilation by activating KATP channels in vascular smooth muscles via PKA-dependent signaling pathway
Autor: | Shuo Chen, Xuewei Hao, Ping Zhang, Hongyang Chen, Lei Yu, Weiwei Cao, Daling Zhu |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
biology Endothelium Activator (genetics) business.industry Vasodilation Cell Biology Pharmacology biology.organism_classification Biochemistry Gastrodia elata 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure chemistry Anesthesia medicine Patch clamp Gastrodin Signal transduction business Molecular Biology Phenylephrine medicine.drug |
Zdroj: | Journal of Receptors and Signal Transduction. 37:543-549 |
ISSN: | 1532-4281 1079-9893 |
Popis: | Gastrodin, one of the major components extracted from the Chinese herb Gastrodia elata Bl., has been widely used as an anticonvulsant, sedative, analgesic and hypotensive. In our study, we aimed to investigate the effects and possible mechanisms of gastrodin on vascular KATP channels. Tension experiments were used on rat mesenteric artery rings without an endothelium. Patch clamp experiments were executed to investigate the influences of gastrodin on the membrane current in mesenteric artery smooth muscle cells. Gastrodin induced vasorelaxation in a concentration dependent manner when rat mesenteric artery rings were pre-contracted with Phenylephrine. The vasorelaxation effect was partially diminished by pre-treating with a KATP channel inhibitor, or a PKA inhibitor. With whole-cell patch-clamp recording techniques, we found that gastrodin is a activator of KATP in rat mesenteric artery smooth muscle cells, and this effect was eliminate by pre-treating with H89or PKI, PKA inhibitor. In addition, w... |
Databáze: | OpenAIRE |
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