A MODIFIED HPLC METHOD FOR RAPI DETECTION OF MOCLOBEMIDE AND IT N-OXIDE METABOLITE IN HUMAN URIN

Autor:	Janelle M. Hoskins, Gillian M. Shenfield, Annette S. Gross
Rok vydání:	2001
Předmět:	Chromatography Elution Metabolite Clinical Biochemistry Pharmaceutical Science Reversed-phase chromatography Urine Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Moclobemide medicine Sample preparation Quantitative analysis (chemistry) medicine.drug
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 24:521-529
ISSN:	1520-572X 1082-6076
DOI:	10.1081/jlc-100103390
Popis:	A rapid and simple high performance liquid chromatographic (HPLC) method to measure moclobemide and its N-oxide metabolite (Ro12-5637) in human urine has been developed by modifying an existing HPLC technique. Sample preparation involves mixing urine with water and internal standard (Ro11-9900) followed by centrifugation. Separation of the analytes in the supernatant was achieved using a reversed-phase Spherisorb C6 column (Phenomenex, 5μm, 250 × 4.6 mm i.d.) eluted with methanol-triethylamine-phosphate buffer (pH7.0). Moclobemide and Ro12-5637 were detected by UV absorption at 240 nm. The limits of quantification for moclobemide and Ro12-5637 were 0.5 and 2.0 μg/mL, respectively. This method has been used to measure the concentrations of moclobemide and Ro12-5637 in urine samples from healthy Caucasians administered a 300 mg oral dose of moclobemide.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6ccbf3443cf53fb57fae468d3d2d9cec https://doi.org/10.1081/jlc-100103390 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.