Improved Extraction and Complete Mass Spectral Characterization of Steroidal Alkaloids from Veratrum Californicum
| Autor: | Susana T. Jiménez, Owen M. McDougal, Katherine V. Ambrose, Ashley A. Fisher, Jeffrey W. Habig, Christopher M. Chandler |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Chromatography Cycloposine Veratrum californicum biology Chemistry Stereochemistry organic chemicals Electrospray ionization Plant Science General Medicine Mass spectrometry Tandem mass spectrometry biology.organism_classification High-performance liquid chromatography Solvent Complementary and alternative medicine Drug Discovery Veratramine heterocyclic compounds |
| Zdroj: | Natural Product Communications. 8:1934578X1300800 |
| ISSN: | 1555-9475 1934-578X |
| DOI: | 10.1177/1934578x1300800806 |
| Popis: | Four steroidal alkaloids extracted from the roots and rhizomes of Veratrum californicum were separated by high performance liquid chromatography (HPLC) and identified using high resolution electrospray ionization time of flight tandem mass spectrometry (ESI-TOF-MS/MS) as veratrosine, cycloposine, veratramine, and cyclopamine. This paper compares ethanol and benzene as extraction solvents, HPLC solvent conditions leading to good separation of steroidal alkaloids, and MS/MS fragmentation patterns for the four steroidal alkaloids which have been released to the public database MassBank.jp. The reported Soxhlet extraction method nearly triples the recovery of steroidal alkaloids from V. californicum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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