Stroma-Free Hemoglobin Solutions Purified by Chloroform and Pasteurization
| Autor: | T I Pristoupil, T Marík, J Suttnar, J Stĕrbíková, Z Tomsová |
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| Rok vydání: | 1990 |
| Předmět: |
Sucrose
Chloroform Isoelectric focusing Sodium 030232 urology & nephrology Biomedical Engineering Medicine (miscellaneous) chemistry.chemical_element Bioengineering Fructose General Medicine 030204 cardiovascular system & hematology Plasma Substitutes Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Biochemistry Hemoglobin Polyacrylamide gel electrophoresis |
| Zdroj: | The International Journal of Artificial Organs. 13:383-387 |
| ISSN: | 1724-6040 0391-3988 |
| DOI: | 10.1177/039139889001300610 |
| Popis: | Several approaches to the processing of native stroma-free hemoglobin solutions (SFH) were reconsidered regarding present requirements for SFH production and quality. Treatment of outdated red blood cells (RBCs) with chloroform and/or by pasteurization were evaluated for technical ease, speed and efficacy in removing stromata, phospholipids and non-heme proteins from RBC hemolysates. The influence of both procedures upon spontaneous hemiglobin formation in stored, preferably freeze-dried SFH was compared. Among other analytical methods, sodium dodecylsulphate Polyacrylamide gel electrophoresis (SDS PAGE) and isoelectrofocusing were used for mutual comparison of the purification procedures. Pasteurized samples were significantly better purified, more homogeneous but also more susceptible to spontaneous oxidation, probably due to heat inactivation of enzymic scavengers of oxygen radicals. On the other hand, the chloroform-treated, unheated SFH samples were less purified from non-heme proteins, but were more stable. Fructose and sucrose were equally active in protecting SFH from oxidation during freeze-drying. At present, the easy chloroform treatment and freeze-drying of thus purified SFH with fructose of sucrose seems to offer a plausible technological compromise which merits further investigation. |
| Databáze: | OpenAIRE |
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