Structural characterization of Ginkgo biloba L. leaves cyclopeptides and anti-inflammatory potency evaluation in human umbilical vein endothelial cells
| Autor: | Huailing Wang, Xianghui Kong, Cuicui Zhang, Man Zheng, Qingwei Liu, Jie Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
biology
010405 organic chemistry Chemistry medicine.drug_class Ginkgo biloba Inflammation Plant Science Pharmacology biology.organism_classification 01 natural sciences Biochemistry Anti-inflammatory Umbilical vein 0104 chemical sciences 010404 medicinal & biomolecular chemistry medicine Potency medicine.symptom Cell adhesion Agronomy and Crop Science Peptide sequence Intracellular Biotechnology |
| Zdroj: | Phytochemistry Letters. 39:12-18 |
| ISSN: | 1874-3900 |
| Popis: | The anti-tumor activity of ginsenosides has been widely explored, however, there is very little research on the anti-inflammatory potency of Ginkgo biloba L. leaves cyclopeptides (GBLCs). In the current study, four novel GBLCs were isolated and their amino acid sequence was determined. Herein, we found that GBLCs alleviated benzo(a)pyrene (BaP)-induced inflammation in human umbilical vein endothelial cells (HUVECs). Interestingly, GBLC-4 [Cyclo-(Leu-Phe-Ile-his)] significantly attenuated the BaP-induced reactive oxygen species generation and mitochondrial membrane potential collapse in HUVECs. Furthermore, GBLC-4 attenuated the expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1, and blocked NF-κB pathway activation to mitigate the injuries caused by BaP. Notably, we provide a novel mechanism, suggesting that GBLCs may be potential agents for vascular inflammatory disease therapy. |
| Databáze: | OpenAIRE |
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