An electrochemiluminescence assay for the detection of bio threat agents in selected food matrices and in the screening of Clostridium botulinum outbreak strains associated with type A botulism
| Autor: | Shashi Sharma, Amita Sachdeva, Ajay K. Singh |
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| Rok vydání: | 2013 |
| Předmět: |
endocrine system
Nutrition and Dietetics biology Clostridium botulinum type A Enterotoxin biology.organism_classification medicine.disease_cause medicine.disease Virology Microbiology Bacillus anthracis medicine Electrochemiluminescence Clostridium botulinum Food microbiology Botulism Multiplex Agronomy and Crop Science Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 94:707-712 |
| ISSN: | 0022-5142 |
| DOI: | 10.1002/jsfa.6310 |
| Popis: | BACKGROUND Specific screening methods for complex food matrices are needed that enable unambiguous and sensitive detection of bio threat agents (BTAs) such as Bacillus anthracis spores and microbial toxins (e.g. staphylococcal enterotoxin B (SEB) and clostridial botulinum neurotoxins (BoNTs)). The present study describes an image-based 96-well Meso Scale Discovery (MSD) electrochemiluminescence (ECL) assay for simultaneous detection of BTAs in dairy milk products. RESULTS The limit of detection of this ECL assay is 40 pg mL−1 for BoNT/A complex, 10 pg mL−1 for SEB and 40 000 CFU mL−1 for Bacillus anthracis spores in dairy milk products. The ECL assay was successfully applied to screen type A Clostridium botulinum outbreak strains. CONCLUSION The results of the study indicate that this ECL assay is very sensitive, rapid ( |
| Databáze: | OpenAIRE |
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