Autor:	T. V. Fedorov, V. I. Surovtsev, M. A. Borozdina, V. Z. Pletnev, V. V. Gusev
Rok vydání:	2003
Předmět:	Staphylococcus simulans biovar staphylolyticus chemistry.chemical_classification Glycylglycine Chromatography biology Lysostaphin Chemistry Biovar General Medicine biology.organism_classification Biochemistry Endopeptidase chemistry.chemical_compound Enzyme Bioorganic chemistry
Zdroj:	Biochemistry (Moscow). 68:50-53
ISSN:	0006-2979
DOI:	10.1023/a:1022141317782
Popis:	This work presents a method for purification of lysostaphin, a glycylglycine endopeptidase, from the culture liquid of S. simulans biovar staphylolyticus to homogeneity in a few steps. The method includes ultrafiltration and ion-exchange and hydrophobic chromatographies. The enzyme was isolated in preparative amounts with the yield of 51%. Some physical and chemical properties of the enzyme are described.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6c9148d191f9b798a61770e0e382e33b https://doi.org/10.1023/a:1022141317782 Zobrazit plný text záznamu Full text from SpringerLink