In vitro Micropropagation of Bacopa monnieri (L.) Penn. - An Important Medicinal Plant
| Autor: | Ahsan Habib, Adnan Rahe, G. M. Al Amin, Ripa Akter Sharmin, Shahina Akter, Mousona Islam, Tanjina Akhtar Banu, Sanjida Rahman Mollika, M. Salim Khan |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Root formation fungi food and beverages Plant Science Biology biology.organism_classification 01 natural sciences Plant tissue Acclimatization In vitro 03 medical and health sciences Horticulture 030104 developmental biology Micropropagation Shoot Bacopa monnieri 010606 plant biology & botany Biotechnology Explant culture |
| Zdroj: | Plant Tissue Culture and Biotechnology. 30:57-63 |
| ISSN: | 1818-8745 1817-3721 |
| Popis: | Investigation on in vitro multiple shoot regeneration in Bacopa monnieri (L.) Penn. using leaf and nodal explants was carried out on MS containing various concentrations and combinations of BAP, Kn, NAA and 2,4-D. Of the two explants, leaf showed the best response towards shoot regeneration and subsequent plant development on MS with 1.0 mg/l BAP and 0.25 mg/l Kn. In this combination, the mean number of shoots/explant was 10.6 ± 0.11 in leaf and 9.6 ± 0.29 in nodal explants. Maximum shoot length was recorded as 12.6 ± 0.21 and 11.20 ± 0.30 from leaf and nodal explants after six weeks of culture, respectively. Half strength of MS supplemented with 0.25 mg/l IBA was found to be the best medium for root formation. The in vitro regenerated plantlets were successfully transplanted in soil after acclimatization. Plant Tissue Cult. & Biotech. 30(1): 57-63, 2020 (June) |
| Databáze: | OpenAIRE |
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