| Autor: |
Wong, Derek, Sogerer, Lisa, Lee, Samantha S., Wong, Victor, Lum, Amy, Levine, Adrian B., Marra, Marco A., Yip, Stephen |
| Předmět: |
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| Popis: |
Additional file 2: Figure S2 ERK dysregulates CIC function. A) Representative Western blot of ATXN1LWT (NHA) and ATXN1LKO (B82) cell lines treated with FGF/EGF over 0-24 hours following serum starvation. FBS control was cultured in FBS for the duration of the timecourse. B) Representative Western blot of HOG cell line treated with FGF/EGF over 0-24 hours following serum starvation. FBS control was cultured in FBS for the duration of the timecourse. C) Representative Western blot of ATXN1LWT (NHA) and ATXN1LKO (B82) cell lines treated with FGF/EGF and/or MEK/ERK inhibitors trametinib/LY3214996. D) Relative mRNA expression of ATXN1L, CIC, and CIC target genes DUSP6, SPRY4, and ETV1/4/5 in ATXN1LWT (HEK) and ATXN1LKO (A30) cell lines treated with FGF/EGF for 8 hours following serum starvation. Gene expression was normalized to TBP and the serum starved parental ATXN1LWT (HEK) cell line was used as a relative control. E) Relative mRNA expression of ATXN1L, CIC, and CIC target genes DUSP6, SPRY4, and ETV1/4/5 in HOG cell line treated with FGF/EGF for 8 hours following serum starvation. Gene expression was normalized to TBP and the serum starved parental ATXN1LWT (HEK) cell line was used as a relative control. * RT-qPCR quantifications were collected from 3 independent experiments. Error bars represent one standard deviation. p-values were calculated using the two-tailed independent Student’s t-test. Statistically significant values are denoted (* = p < 0.05, ** = p < 0.01). Individual data values can be found in Additional file 17: Table S10. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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