The Evaluation of Biofilm-Forming Potential of Escherichia coli Collected From the Equine Female Reproductive Tract

Autor: D.P. Beehan, Dale L. Paccamonti, Natali Krekeler, Karen Wolfsdorf, Justine Elam, S.K. Lyle
Rok vydání: 2015
Předmět:
Zdroj: Journal of Equine Veterinary Science. 35:935-939
ISSN: 0737-0806
DOI: 10.1016/j.jevs.2015.08.018
Popis: Escherichia coli is one of the most common microbial pathogens of equine endometritis. Despite seemingly appropriate veterinary intervention, these infections can become chronic in some mares resulting in long-term infertility. The formation of bacterial biofilm within the uterus has become a recently theorized, yet unsubstantiated, explanation for unresponsive endometrial bacterial infections in mares. The objective of this study was to examine the biofilm-forming potential (BFP) of E. coli isolates collected from the female equine reproductive tract by using the crystal violet assay and scanning electron microscopy. Approximately 27% of isolates (35/130) demonstrated strong BFP, with 23% (19/81) and 33% (16/49) of uterine and clitoral fossa isolates, respectively, showing strong BFP. Scanning electron microscopy showed E. coli arranged in a community of cells surrounded by an extracellular matrix of polymeric substances. Our results have shown a large number of E. coli isolates display BFP. Our results strongly support the theory that biofilm formation may be an important cause of chronic endometrial infection.
Databáze: OpenAIRE
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