| Autor: |
Takako Murayama, Woon Joo Lee, Yumiko Ikegami, Rieko Kawanami, R Nawada, Ryoichi Amitani |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
Thorax. 53:975-978 |
| ISSN: |
0040-6376 |
| DOI: |
10.1136/thx.53.11.975 |
| Popis: |
BACKGROUND—Aspergillus fumigatus can colonise the airways and the lungs with localised underlying conditions and occasionally invade the surrounding lung tissues even in subjects without systemic predisposing factors, presumably by escaping the local host defences. The aim of this study was to investigate the effects of A fumigatus culture filtrate (ACF) on the activities of human phagocytes—inhibition of germination of A fumigatus spores by alveolar macrophages (AMs) and hyphal damage by polymorphonuclear leucocytes (PMNs)—which are the critical host defences against A fumigatus. METHODS—Spores were incubated with AMs at a ratio of 1:1 in a medium containing different concentrations of ACF for 10 hours at 37°C. Spore germination was visualised with light microscopy and the inhibition rate was calculated. The percentage of hyphal damage caused by PMNs pretreated with various concentrations of ACF was measured by a colorimetric tetrazolium metabolic assay. RESULTS—The inhibition rate of spore germination by AMs cultured with medium alone (control) was 90 (0.8)% whereas that by AMs cultured with the medium containing 10% ACF was significantly (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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