One-step, visual and sensitive detection of phorate in blood based on a DNA–AgNC aptasensor
| Autor: | Jian Shi, Yadong Guo, Yanjun Ding, Lingmei Lan, Yunfeng Chang, Jifeng Cai, Leiming Han, Ceng Chen, Xingmei Li, Weichen Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Chromatography Phorate Chemistry Aptamer 010401 analytical chemistry Nanoprobe One-Step 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology 01 natural sciences Catalysis 0104 chemical sciences chemistry.chemical_compound Clinical diagnosis Materials Chemistry A-DNA Gas chromatography 0210 nano-technology |
| Zdroj: | New Journal of Chemistry. 42:6293-6298 |
| ISSN: | 1369-9261 1144-0546 |
| DOI: | 10.1039/c8nj00958a |
| Popis: | The ingestion of organophosphate (Op) pesticides for attempting suicide has been a serious health issue recently. Phorate, a class of Op, has attracted attention in this regard and so the rapid diagnosis and detection of phorate is crucial for human health. In this study, a one-step and visual method for the rapid determination of phorate via an aptasensor based on DNA-templated silver nanoclusters (DNA–AgNCs) has been presented. The designed DNA–AgNC nanoprobe, containing a special structure with an intercalated aptamer, shows excellent stability. Introducing phorate to the system easily triggers the aggregation of DNA–AgNCs, producing a brown-to-colorless color change in the solution in only 6 minutes. Under the optimal conditions, the aptasensor can allow for the determination of phorate in the concentration range of 0–25 μg mL−1, using two linear equations: y = −2.308x + 0.996 (R2 = 0.977) for 0–0.125 μg mL−1 and y = −0.020x + 0.664 (R2 = 0.971) for 0.125–25 μg mL−1. The estimated detection limit of phorate was 0.012 ng mL−1. Moreover, the practicability of this proposed method was also validated by analyzing phorate-spiked human blood samples and the results were in agreement with those from gas chromatography/mass spectrometry (GC/MS) analysis. Therefore, this fabricated aptasensor is suitable for sensing phorate in complex biological samples, and shows high potential for clinical diagnosis or forensic toxicology analysis. |
| Databáze: | OpenAIRE |
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