Multicenter evaluation of enhanced chemiluminescence labeled-antibody immunoassay (Amerlite-MAB) for free thyroxine
| Autor: | N D Christofides, C P Sheehan |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
medicine.diagnostic_test biology business.industry medicine.drug_class Biochemistry (medical) Clinical Biochemistry Reference range Free thyroxine Monoclonal antibody Gastroenterology law.invention Endocrinology law Immunoassay Internal medicine Nonthyroidal illness biology.protein Medicine Euthyroid Antibody business hormones hormone substitutes and hormone antagonists Chemiluminescence |
| Zdroj: | Clinical Chemistry. 41:24-31 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/41.1.24 |
| Popis: | The technical and diagnostic performance of the Amerlite-MAB enhanced luminescence assay for free thyroxine (FT4) was assessed in a multicenter evaluation trial. The euthyroid central 95% reference range for FT4 (1393 subjects) was 11.5-27.7 pmol/L (by cumulative frequency plot with two-tailed 2.5% cutoffs). Results (y) agreed with those of similar radioactive method (Amerlex-MAB FT4) (x): y = 1.06 x + 0.54, Sylx = 335.5, n = 235). Mean within-assay precision (CV) in six centers was 5.7% at 6 pmol/L and 2.6% at 51 pmol/L FT4; between-assay precision (CV) was 7.2% and 3.6%, respectively. Diagnostic performance was assessed in all patient groups usually encountered, including those with nonthyroidal illness and extreme binding-protein anomalies. In elderly euthyroid subjects, the proportion of above-normal FT4 values exceeded that in younger age-groups. These increases often accompanied normal estimates of thyrotropin and, in some cases, might have arisen by interference from medication that is taken more often by the elderly. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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