| Autor: |
Joan Hauser, Steve Menchen, Pete Theisen, Scott C. Benson, Krishna G. Upadhya, Paul M. Kenney |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
SPIE Proceedings. |
| ISSN: |
0277-786X |
| DOI: |
10.1117/12.380498 |
| Popis: |
Labeling of DNA with fluorescent reporter is generally accomplished by attaching fluorescent dyes to the 5'-end of primers for PCR based applications, including geno-typing and linkage mapping. Requirements of spectral resolution and chemical stability have guided us toward asymmetric fluorescein dye structures whose chemical and physical properties have been fine-tuned through substituent modification. We have developed dyes that are amenable to 5'-prime labeling on solid support as dye-amidites facilitating automated dye-primer synthesis.© (2000) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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