032 Co-transformation with Two Vectors and Regeneration of Transgenic Plants on the Base of High-efficient Agrobacterium-mediated Gene Transfer in Grape Embryogenic Cell Suspension

Autor:	Sylvie Even, Avi Perl, Sharon Farchie, Violeta Colova-Tsolova, Nahman Shahar, Rachel Gollop
Rok vydání:	2000
Předmět:	Transformation (genetics) biology Agrobacterium Regeneration (biology) fungi Botany food and beverages Gene transfer Genetically modified crops Horticulture biology.organism_classification Suspension culture Cell biology
Zdroj:	HortScience. 35:393D-393
ISSN:	2327-9834 0018-5345
DOI:	10.21273/hortsci.35.3.393d
Popis:	Embryogenic cell suspension was developed from in vivo anthers of seedless grape cv. Sugarone. Agrobacterium genetic co-transformation was realized with two vectors carried respectively two different reporter genes: hpt and nptII, and three (1+2) agronomicaly beneficial genes encoding for proteins that are involved in fungus disease resistance. The effciency of transformation procedure and integraton of foreign genes was verified by hystochemical assay as a first step after insertion in embryogenic suspension two different constructs with Gus-reporter gene under control of different promotors. PCR assay and Southern blot analysis were used to confirm the co-transformation in regenerated grape plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6c1924b19d5762a16a48452ac721561f https://doi.org/10.21273/hortsci.35.3.393d Zobrazit plný text záznamu