| Autor: |
Martin Hof, K Krämer, R. Hutterer, Friedemann W. Schneider |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Chemistry and Physics of Lipids. 90:11-23 |
| ISSN: |
0009-3084 |
| DOI: |
10.1016/s0009-3084(97)00087-x |
| Popis: |
Fluorescence quenching and resonance energy transfer have been used to determine the localization of the local anesthetic tetracaine in vesicles composed of 1,2-dimyristoyl- sn -glycero-3-phosphatidylcholine (DMPC) as a function of both temperature and ionic strength. The fluorescence behaviour of tetracaine in vesicles can be attributed to its different partition coefficients in acid and basic solution, in gel phase and fluid phase vesicles, respectively. Using both steady-state and time-resolved fluorescence measurements we show that a saturable binding rather than a partitioning model holds for the interaction of tetracaine with gel phase bilayers. The relative quenching efficiencies of the series of n -AS dyes depend on the phase state of the bilayer and suggest a deeper incorporation of tetracaine in fluid phase than in gel phase membranes. Resonance energy transfer measurements support the view that tetracaine is incorporated predominantly in the region of the 9-AS chromophore in DMPC-bilayers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect