CHEMOSENSITIZATION OF BLADDER CARCINOMA CELLS BY BCL-xL ANTISENSE OLIGONUCLEOTIDES

Autor:	C. A. Stein, Joshua Ojwang, Irina V. Lebedeva, Robert R. Rando, Anthony J. Raffo, Paul A. Cossum
Rok vydání:	2001
Předmět:	Messenger RNA biology Chemosensitization Cell culture Apoptosis Annexin Urology biology.protein Cancer research Bcl-xL Viability assay Northern blot urologic and male genital diseases
Zdroj:	Journal of Urology. 166:461-469
ISSN:	1527-3792 0022-5347
DOI:	10.1016/s0022-5347(05)65964-2
Popis:	Purpose: We investigated antisense inhibition of anti-apoptotic bcl-xL and bcl-2 proteins to increase chemosensitization in the T24 and 5637 bladder carcinoma cell lines.Materials and Methods: A T24 bladder carcinoma cell line stably over expressing bcl-xL protein was constructed. Apoptosis by cytotoxic agents was estimated by cell cycle analysis and Annexin V binding. To eliminate bcl-xL expression T24 and 5637 cells were treated with C5-propynylated and 2′-O-methylribo-oligonucleotides. Levels of protein and messenger RNA were measured by Western and Northern blot analysis. Cell viability after combined treatment with oligonucleotides and various cytotoxic agents was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and evaluated statistically by Student’s 2-sample t test.Results: Forced over expression of bcl-xL protein desensitized the T24 bladder carcinoma cell line to cytotoxic agents. C5-propynylated and 2′-O-methylribo-oligonucleotides down-regulated bcl-xL protein exp...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6bfc3f89edc7c11f83af64b84cbae198 https://doi.org/10.1016/s0022-5347(05)65964-2 Zobrazit plný text záznamu