Studies Of The Logic Of Electron Transfer In Oxidoreductase Enzymes

Autor:	R. Pethig, Frederick F. Becker, M.H. Capstick, Peter R. C. Gascoyne
Rok vydání:	2005
Předmět:	chemistry.chemical_classification Electron transfer Nitroxide mediated radical polymerization Nuclear magnetic resonance Membrane Semiquinone Chemistry Oxidoreductase Radical Electron acceptor Proton-coupled electron transfer Photochemistry
Zdroj:	[1990] Proceedings of the Twelfth Annual International Conference of the IEEE Engineering in Medicine and Biology Society.
Popis:	An electrodeless method is described for measuring the intrinsic electron transfer processes that occur in oxidoreductase enzymes. The enzymes may be in free solution, immobilized in membranes, or exist in optically opaque media. Electron injection into the enzyme is accomplished chemically using NAD(P)H. and semiquinone or nitroxide free radicals act as electron acceptors. The rate of electron transfer through the enzyme is determined by monitoring the decay of the radicals using an electron spin resonance spectrometer, and from analyses of the decay kinetics the corresponding logic state diagrams are derived.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6bf9a61692e5766f1d9ede53262f2a50 https://doi.org/10.1109/iembs.1990.691984 Zobrazit plný text záznamu