In vitro irradiation of doxorubicin with 18F-FDG Cerenkov radiation and its potential application as a theragnostic system
| Autor: | Eugenio Torres-García, Miguel A. Camacho-López, Enrique Morales-Avila, Keila Isaac-Olivé, Myrna Luna-Gutiérrez, Liliana Aranda-Lara, Mariana Sánchez-Holguín, Ninfa Ramírez-Durán, Hilda Angeline Quintos-Meneses |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Radiation Radiological and Ultrasound Technology Nuclear imaging Chemistry medicine.medical_treatment 030303 biophysics Biophysics Photodynamic therapy 02 engineering and technology 021001 nanoscience & nanotechnology In vitro 03 medical and health sciences medicine Cytotoxic T cell Radiology Nuclear Medicine and imaging Doxorubicin Irradiation 0210 nano-technology Cytotoxicity Laser beams medicine.drug |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 210:111961 |
| ISSN: | 1011-1344 |
| Popis: | Doxorubicin (DOX), an effective chemotherapeutic agent, has a wide excitation band centred at 480 nm. Cerenkov radiation (CR) is considered an internal light source in photodynamic therapy (PDT). DOX could be photoactivated by CR and thus, enhancing its cytotoxicity. In this work, 18F-FDG was used to evaluate the effect of Cerenkov radiation on DOX, in comparison to irradiation with a 450-nm laser beam, in terms of ROS production. The production of 1O2 and O2⁎- reactive species during DOX irradiation was detected indirectly by ABMA and DCPIP bleaching, respectively. The cytotoxic effect of the DOX / 18F-FDG CR system was evaluated in the T47D breast cancer cell line. The irradiation of DOX produced 1O2 and O2⁎- species using both 18F-FDG CR and a 450-nm laser beam. The majority reactive species produced in both cases was 1O2; a favourable result, given the greater cytotoxicity of this species. The viability of T47D cells in presence of DOX (5 nM), 18F-FDG (37.5 μCi) and DOX (5 nM)/18F-FDG (37.5 μCi) was (86 ± 9)%, (84 ± 8)% and (64 ± 5)%, respectively; these results suggest a synergistic cytotoxic effect derived from the cytotoxic activity of DOX and its photoactivation by 18F-FDG CR. It is worth noting that the system could be optimized in terms of DOX concentration and 18F-FDG activity for better results. Due to the fact that 18F-FDG is widely used in nuclear imaging, the DOX/18F-FDG system also possesses theragnostic characteristics. Thus, in this work, it is demonstrated that DOX can be used in a dual therapy system based on chemotherapy-PDT when 18F-FDG CR is used as a DOX excitation source. |
| Databáze: | OpenAIRE |
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