The Effects of Cyclosporin-A on Human Vascular Smooth Muscle Cells Apoptosis

Autor:	C. Wei., J. S. McLaughlin, M. Kinjo
Rok vydání:	2000
Předmět:	Vascular smooth muscle Apoptosis business.industry Cyclosporin a Cancer research Medicine business Instrumentation
Zdroj:	Microscopy and Microanalysis. 6:634-635
ISSN:	1435-8115 1431-9276
DOI:	10.1017/s1431927600035662
Popis:	Cyclosporine-A is the one of the most effective immunosuppressant drug in organ transplantation. However, cyclosporine-A has a potent cytotoxic effect. Cellular apoptosis is being suggested may contribute to the cyclosporine A-mediated cytotoxic action. To date, regarding the effects of cyclosporine-A on apoptosis and apoptosis-related genes expression in vascular smooth muscle cells remain unclear. Therefore, the current study was designed to investigate the actions of cyclosporine-A on apoptosis and p53 expression in human vascular smooth muscle cells.Human saphenous vein was obtained from coronary artery bypass surgery (n=5). After saphenous vein removed, the tissue was minced and incubated in the special tissue culture system for 24 hours in the absence or presence of cyclosporine-A (10-6M). To detect the DNA fragmentation, in situ terminal deoxymucleotidyl transferase dUTP nick end labeling (TUNEL) was performed. The p53 level in human vascular smooth muscle cells was determined by immunohistochemical staining (IHCS). An average of 1000 nuclei was analyzed for both TUNEL and p53 EHCS studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6bcf87cd347b78917d1fb6bc79e2a9e3 https://doi.org/10.1017/s1431927600035662 Zobrazit plný text záznamu