| Popis: |
Benzoyl chloride derivatization of dissolved metabolites in seawater and culture medium improves sensitivity, number of quantifiable compounds, and sample throughput. Samples are quantified using a standard curve and stable isotope labeled internal internal standards prepared from an isotopically labeled reagent. Samples are filtered (0.2 um), derivatized in basic solution and extracted from seawater/culture medium using a solid phase extraction resin (PPL, Bond Elut, Agilent). Benzoylated metabolites are detected using UPLC-MSMS with Orbitrap detection. Peaks are analyzed with Skyline. |
