Interaction of Escherichia coli O157:H7 E318 cells with the mucus of harvested channel catfish (Ictalurus punctatus)

Autor:	Jinru Chen, Yao-Wen Huang, Rico R. Suhalim
Rok vydání:	2007
Předmět:	food.ingredient biology respiratory system biology.organism_classification medicine.disease_cause Mucus Enterobacteriaceae Microbiology fluids and secretions food Ictalurus medicine Agar Incubation Escherichia coli Bacteria Food Science Catfish
Zdroj:	LWT - Food Science and Technology. 40:1266-1270
ISSN:	0023-6438
DOI:	10.1016/j.lwt.2006.08.017
Popis:	Channel catfish skin with or without mucus (0.5 cm in diameter) were immersed into a suspension containing 10 9 CFU/ml of Escherichia coli O157:H7 E318 cells at 22 °C for 20 min. The inhibitory effect of skin mucus was determined by placing the mucus-side down on tryptic soy agar inoculated with 10 4 –10 5 CFU of E. coli O157:H7 E318. The inhibition zones of fish mucus had a diameter of approximately 0.7 cm and were only visible for the first 12 h of the incubation. Bacterial cells were observed at 15 μm into the mucus layer under confocal scanning laser microscopy (CSLM). Plate counts and CSLM revealed 0.5- and 1-log less cells, respectively, attached to skin without mucus than to skin with mucus. Results suggest that E. coli O157:H7 E318 could attach to and penetrate through the mucus of channel catfish and may become a source of contamination during catfish processing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6bc28251149469205b593ad07c322315 https://doi.org/10.1016/j.lwt.2006.08.017 Zobrazit plný text záznamu Full Text from ScienceDirect