Isolation of microsatellites via enrichment and a sequence tagged method in a South American lizard with suspected parental care, Liolaemus leopardinus

Autor:	Michael G. Gardner, Stanley F. Fox, Andrew R. Murphy
Rok vydání:	2009
Předmět:	Genetics education.field_of_study Linkage disequilibrium Population Locus (genetics) Liolaemus leopardinus Biology biology.organism_classification Loss of heterozygosity Microsatellite Allele education Microsatellite enrichment Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics Resources. 1:13-16
ISSN:	1877-7260 1877-7252
DOI:	10.1007/s12686-009-9003-1
Popis:	We used an enrichment and a sequence tagged microsatellite method (STM) to isolate 11 novel di and tri microsatellites for the high altitude living lizard Liolaemus leopardinus. These loci were trialled in 61 individuals from a single population. Seven loci conformed to Hardy–Weinberg expectations and were in linkage equilibrium. The 7 loci displayed intermediate levels of polymorphism exhibiting between 2 and 10 alleles per locus, with observed heterozygosity values ranging from 0.328 to 0.745. These markers will be useful in population level analyses and in the potential identification of complex social structure and parental care in this species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6bacf464bf819f236edfb35509c4caea https://doi.org/10.1007/s12686-009-9003-1 Zobrazit plný text záznamu